Regulatory

Part:BBa_K415200

Designed by: Grant Robinson   Group: iGEM10_MIT   (2010-08-20)


Strong Constitutive T5 Promoter with cmt Operator (T5-cmt)

This is a strong, constitutive partial T5 promoter with the cmt operator appended immediately afterward. This construct was specified in a recent (as of 2010) paper by Choi, et al., titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains." In the absence of cymR, this promoter should function as a strong, constitutive promoter; in the presence of cymR, this promoter should be repressed with essentially zero leaky transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None